total cellular protein extraction reagent Search Results


yeast total cellular protein extracts  (Kamada)
90
Kamada yeast total cellular protein extracts
Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote <t>yeast</t> mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total <t>cellular</t> <t>protein</t> <t>extracts</t> were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909
Yeast Total Cellular Protein Extracts, supplied by Kamada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast total cellular protein extracts - by Bioz Stars, 2026-04
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total cellular protein extraction reagent  (Keygen Biotech)
90
Keygen Biotech total cellular protein extraction reagent
Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote <t>yeast</t> mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total <t>cellular</t> <t>protein</t> <t>extracts</t> were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909
Total Cellular Protein Extraction Reagent, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total cellular protein extraction reagent/product/Keygen Biotech
Average 90 stars, based on 1 article reviews
total cellular protein extraction reagent - by Bioz Stars, 2026-04
90/100 stars
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total cellular protein extracts  (Epizyme Inc)
90
Epizyme Inc total cellular protein extracts
Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote <t>yeast</t> mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total <t>cellular</t> <t>protein</t> <t>extracts</t> were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909
Total Cellular Protein Extracts, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total cellular protein extracts/product/Epizyme Inc
Average 90 stars, based on 1 article reviews
total cellular protein extracts - by Bioz Stars, 2026-04
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cellular protein extraction reagent a supplemented with phenylmethylsulfonyl fluoride (pmsf)  (Beyotime)
90
Beyotime cellular protein extraction reagent a supplemented with phenylmethylsulfonyl fluoride (pmsf)
Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote <t>yeast</t> mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total <t>cellular</t> <t>protein</t> <t>extracts</t> were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909
Cellular Protein Extraction Reagent A Supplemented With Phenylmethylsulfonyl Fluoride (Pmsf), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellular protein extraction reagent a supplemented with phenylmethylsulfonyl fluoride (pmsf)/product/Beyotime
Average 90 stars, based on 1 article reviews
cellular protein extraction reagent a supplemented with phenylmethylsulfonyl fluoride (pmsf) - by Bioz Stars, 2026-04
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cellular protein extraction reagent b  (Beyotime)
90
Beyotime cellular protein extraction reagent b
Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote <t>yeast</t> mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total <t>cellular</t> <t>protein</t> <t>extracts</t> were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909
Cellular Protein Extraction Reagent B, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellular protein extraction reagent b/product/Beyotime
Average 90 stars, based on 1 article reviews
cellular protein extraction reagent b - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote yeast mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total cellular protein extracts were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909

Journal: Yeast (Chichester, England)

Article Title: Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes

doi: 10.1002/yea.1709

Figure Lengend Snippet: Characterization of the translation start site of Ste23p. (A) Schematic of the genomic DNA sequence that is 5′ to the predicted start codon of the STE23 open reading frame within pWS482. Schematic is not drawn to scale. (B) The predicted 5′ UTR of STE23 is not required for expression of functional Ste23p. A set of plasmids bearing deletions of the 5′ UTR contained within pWS482 (pWS759-763) and a plasmid lacking part of the STE23 ORF itself (pWS765) were evaluated for their ability to promote yeast mating (top panel) and Ste23p expression (middle panel). Yeast mating was evaluated by mixing MATα cells (IH1793) and M16A-deficient MATa cells (Y272) carrying the indicated plasmid, followed by selection on diploid-selective media. Ste23p expression was evaluated by immunoblotting. Equivalent percentage amounts (2%) of total cellular protein extracts were separated by SDS-PAGE and transferred onto blots that were probed with anti-HA and anti-Act1p antibodies as a loading control (middle and bottom panels, respectively). (C) Expression of Ste23p partially rescues the mating defect of an M16A-deficient yeast strain (Y272). Yeast mating assays and analysis of Ste23p expression were performed as described in (B); the panel order is preserved. WT (IH1783) was transformed with an empty vector (pRS316), and Y272 was transformed with either pRS316 or a plasmid encoding Ste23p-2HA (pWS482). (D) The annotated start codon of STE23 is not required for expression of functional Ste23p. Yeast mating assays and analysis of Ste23p expression were performed as described in (B), using plasmid-transformed Y272 cells, except that 4% of each extract was evaluated; the panel order is preserved. Plasmids used were pWS908-909

Article Snippet: Yeast total cellular protein extracts were prepared as previously described ( Fujimura-Kamada et al. , 1997 ).

Techniques: Sequencing, Expressing, Functional Assay, Plasmid Preparation, Selection, Western Blot, SDS Page, Control, Transformation Assay

Ste23p is expressed in both haploid and diploid yeast. Plasmid-based expression of Ste23p (A) and Axl1p (B) was examined in MATa (IH1783), MATα (IH1784) and diploid (IH1788) cell types, using pWS482 and pWS371, respectively. The steady-state levels of the indicated HA-tagged protein (top panel) and yeast actin (bottom panel) were detected by immunoblotting as described in Figure 6, except that 60% of the total cellular extract preparation was evaluated in the instance of Axl1p samples, due to its low abundance

Journal: Yeast (Chichester, England)

Article Title: Yeast Ste23p shares functional similarities with mammalian insulin-degrading enzymes

doi: 10.1002/yea.1709

Figure Lengend Snippet: Ste23p is expressed in both haploid and diploid yeast. Plasmid-based expression of Ste23p (A) and Axl1p (B) was examined in MATa (IH1783), MATα (IH1784) and diploid (IH1788) cell types, using pWS482 and pWS371, respectively. The steady-state levels of the indicated HA-tagged protein (top panel) and yeast actin (bottom panel) were detected by immunoblotting as described in Figure 6, except that 60% of the total cellular extract preparation was evaluated in the instance of Axl1p samples, due to its low abundance

Article Snippet: Yeast total cellular protein extracts were prepared as previously described ( Fujimura-Kamada et al. , 1997 ).

Techniques: Plasmid Preparation, Expressing, Western Blot